The SSP, SSO, SBT & NGS molecular HLA typing product lines from One Lambda features the quality, accuracy and innovation of serological typing methods and provides higher resolution results using DNA Technology. One Lambda offers a range of molecular typing technologies, depending on the user’s requirement:

Product Range

SupplierProduct Portfolio
One Lambda

Micro SSP – sequence specific primers, One Lambda’s portfolio of Micro SSP™ typing trays provide complete HLA Class I and Class II DNA typing.Using Generic Trays, technologists can either assign HLA Class I or Class II alleles individually or simultaneously. If more detailed typing is needed, the High Resolution Allele Specific Trays will help eliminate redundancies.

Allset+ Gold SSP AllSet+ Gold sequence specific primer (SSP) kits. Allele specific kits result in fewer ambiguities compared to serology or some sequence oligonucleotide (SSO) methods. A wide selection customized for addressing HLA typing needs:

Direct to higher-resolution for all HLA loci.Higher-resolution for HLA Class I and Class II loci

Low-resolution for HLA Class I and Class II loci, including combination plates for ABC, ABDR, ABDRDQ, and DRDQ

LabType® SSO – reverse sequence specific oligonucleotides, by bead based Multiplexing LABType products are reverse SSO DNA typing assays that provide superior resolution for HLA A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1 HLA typing to be analysed on the LabScan100 or LabScan3D systems The newly added LABType XR and CWD assays, exclusively designed for use with the LABScan3D™, allow multiplexing of up to 500 bead regions allowing for a broader combination of detection probes for a single sample.

Secore SBT Kits SeCore kits are high resolution HLA typing products based on sequence based typing. Our kits are designed for ease of use and yield high quality results. Locus Sequencing Kits provide reagents for amplification, purification, and Sanger sequencing of HLA alleles (variants) from DNA. Sequencing fragments produced using this CE Marked kit are subjected to capillary electrophore.

NGS Solution NGS HLA Typing assays offer first pass, high-resolution genotyping of Class I and Class II loci, using a multiplexed cocktail of primers to amplify targeted genes. Our Class I and Class II kits amplify HLA-A, -B, -C, -DRB1, -DQB1, -DPB1 -DQA1 and -DPA1 loci in four reactions in a single pass. The total NGS solution includes a multiplexed amplification strategy for HLA genes, library and template preparation, the proven Ion PGM sequencing platform, and TypeStream™, which is a state-of-the-art data analysis software

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